blue-white screen

English

blue-white screen (plural blue-white screens)

(biology) A screening technique for the rapid detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector, which is then inserted into a host cell; these are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.

单词	blue-white screen
释义	blue-white screen English Noun blue-white screen (plural blue-white screens) (biology) A screening technique for the rapid detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector, which is then inserted into a host cell; these are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
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