biotinylate

English

Etymology

biotinyl +‎ -ate

Verb

biotinylate (third-person singular simple present biotinylates, present participle biotinylating, simple past and past participle biotinylated)

To attach a biotin residue to a biological macromolecule in order to label it
- 2015 July 11, Eugene J. H. Wee, Thu Ha Ngo, Matt Trau, “Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs”, in Clinical Epigenetics‎, volume 7, DOI:10.1186/s13148-015-0100-6:
  After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.

Related terms

biotinylation

单词	biotinylate
释义	biotinylate English Etymology biotinyl +‎ -ate Verb biotinylate (third-person singular simple present biotinylates, present participle biotinylating, simple past and past participle biotinylated) To attach a biotin residue to a biological macromolecule in order to label it 2015 July 11, Eugene J. H. Wee, Thu Ha Ngo, Matt Trau, “Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs”, in Clinical Epigenetics‎, volume 7, DOI:10.1186/s13148-015-0100-6: After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA. Related terms biotinylation
随便看	perverteixis perverteixo pervertemini pervertemur pervertemus pervertenda pervertendae pervertendam pervertendarum pervertendas pervertende pervertendi pervertendis pervertendo pervertendoci pervertendomi pervertendorum pervertendos pervertendosi pervertendoti pervertendovi pervertendum pervertendus pervertens pervertent